Before: All together, table 1 showed that LC/ESI/MS^n procedure profiled more than 80 phospholipid molecular species from both T. cuspidata and T. chinensis var. mairei cells. Due to effects of different molecular species on instrument response, the absolute quantification of phospholipids has not been performed in our experiments. The relative abundance of individual molecular species within a phospholipids class was calculated according to Materials and Methods. As shown in table 1, major molecular species composition of PC, PE, PS and PA of T. cuspidata at 2 weeks was similar to 2-week T. chinensis var. mairei. For instance, 16:0/20:4 ( 18:1/18:3 and 18:2/18:2 ) , 16:0/20:3 and 18:2/20:4 PE were prevalent in PE species , which constituted 18.0 % , 13.8 % and 9.3 % of total PE species in T. cuspidata, and 18.7 %, 13.3 %, 8.9 % in T. chinensis var. mairei. PS molecular species profiles were much less complex than profiles of PC and PE, and almost dominated by long-chain polyunsaturated fatty acids ( PUFA ) 24:0/20:4 and 22:0/ 20:4 PS in two Taxus species cells. For PG and PI, the most abundant molecular species were 16:0/18:3 and 16:0/18:2, which have some differences in two Taxus species cells ( * P<0.05 ). In addition, special PUFA ( C20:4 or C20:3 ) were relatively abundant in PE and PC species, including 16:0/20:4, 16:0/20:3, 18:2/20:4, 18:1/20:4 , 18:1/20:3 and 18:0/20:4.
	Before: 3.5. Applying to human plasma samples The blood from 10 clinical patients was obtained and the plasma concentration of linomycin determined with the proposed method and the results are shown as below (Table 3). As is shown, linomycin was not detected in most patients. These data suggest that the virtual absence of absorption of this drug, which implies negligible passage into the general circulation. However, this can not be confirmed in the absence of information on the extent of metabolism.